Kano Independent Research Centre Trust (KIRCT) – Genomics Centre
Genomics and Molecular Biology Lab:
This lab is equipped with state-of-the-art equipment for molecular biology techniques such as PCR, sequencing, and gene expression analysis.
Subunits:
- Gene Expression Analysis
- Genotyping
- Sequencing
Mission:
The Genomics Centre at Kano Independent Research Centre Trust (KIRCT) is committed to advancing the field of genomics in Nigeria, with a focus on improving public health and reducing the burden of diseases in the country. Our mission is to apply cutting-edge genomics technologies and expertise to address the pressing health challenges facing Nigeria, particularly in the areas of communicable and non-communicable diseases.
Objectives:
1. To establish a world-class genomics facility in Nigeria, equipped with state-of-the-art equipment and expertise.
2. To conduct research in genomics, focusing on preeclampsia, diabetes and hypertension prevalent in Nigeria.
3. To develop and implement genomic-based diagnostic tools and therapeutic strategies for the prevention and treatment of diseases.
4. To train and mentor local researchers, clinicians, and students in genomics research and its applications.
5. To collaborate with national and international organizations to advance genomics research and translation in Nigeria.
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GENIE II Instrument
- Portable Diagnostic Solution: Utilizes Loop-Mediated Isothermal Amplification (LAMP) for rapid pathogen detection.
- Real-Time Data: Provides immediate results, supporting swift decision-making for health emergencies.
- Field-Ready Design: Ideal for both laboratory and field settings, maximizing versatility.
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HPLC (High-Performance Liquid Chromatography)
- High Precision: Deliver consistent and reproducible results across a wide range of sample types.
- Rapid Separation: Achieve fast and efficient analysis with superior resolution.
- Versatile Applications: Ideal for pharmaceuticals, food safety, environmental testing, and biochemistry research.
- Advanced Detection: Multiple detector options, including UV-Vis, fluorescence, and mass spectrometry.
- User-Friendly Interface: Intuitive software and easy-to-use features simplify operation and data analysis.
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LCMSD (Liquid Chromatography Mass Spectrometry Detector)
- High Sensitivity: Detect and quantify trace-level compounds with exceptional accuracy.
- Precise Separation: Achieve superior resolution for complex mixtures with advanced liquid chromatography.
- Versatile Applications: Ideal for pharmaceuticals, environmental analysis, food safety, and clinical research.
- Rapid Analysis: Streamline workflows with fast run times and high-throughput capabilities.
- User-Friendly Interface: Simplified operation and robust software for data analysis and reporting.
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NanoDrop Oneᶜ UV-Vis Spectrophotometer
- Microvolume Analysis: Measure as little as 1–2 µL of sample with consistent accuracy.
- Integrated Cuvette Capability: Expand versatility with standard cuvette-based measurements.
- Rapid Results: Obtain data in seconds with minimal sample preparation.
- Advanced Contaminant Detection: Identify impurities with built-in spectral analysis for better data integrity.
- User-Friendly Design: Intuitive touchscreen interface and easy data management via Wi-Fi or USB.
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Qubit™ Flex Fluorometer
- Exceptional Sensitivity: Detect even low-concentration samples with fluorescence-based quantification.
- Flexible Format: Analyze up to 8 samples simultaneously for increased throughput.
- Broad Applications: Ideal for DNA, RNA, and protein quantification, as well as other assays.
- Intuitive Design: Touchscreen interface and preloaded protocols for fast, user-friendly operation.
- Sample Conservation: Requires only 1–20 µL of sample, preserving precious resources.
- KingFisher Flex Automated Extraction System
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QuantStudio™ 7 Flex Real-Time PCR System
- High Sensitivity and Accuracy: Detect and quantify even low-abundance targets with precision.
- Flexible Format: Supports multiple block types (96-well, 384-well, and TaqMan® Array Cards) to accommodate diverse sample needs.
- Multiplexing Capability: Analyze multiple targets in a single reaction for greater efficiency.
- Advanced Connectivity: Cloud-enabled system for remote monitoring and data sharing.
- User-Friendly Interface: Intuitive software for streamlined experimental setup and data analysis.
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QuantStudio™ 7 Flex Real-Time PCR System
From gene expression to mutation analysis, the QuantStudio™ 7 Flex revolutionizes real-time PCR applications with precision and flexibility. Its multiplexing capabilities allow simultaneous analysis of multiple targets, supporting complex research endeavours.
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Illumina MiSeq i100 plus
- Fast Turnaround Time: Delivers results up to four times faster than previous models, with sequencing run times as short as four hours, enabling same-day results.
- High Output and Flexibility: It offers an output range of 1.5 to 30 gigabases and supports up to 200 million paired-end reads per run.
- Simplified Workflow: Streamlined workflow with integrated cartridges for library loading, reducing setup to three steps that can be completed in under 20 minutes.
- This design eliminates the need for washes between runs, improving sequencing efficiency.
- Onboard Data Analysis: Preconfigured DRAGEN analysis pipelines are available generating data analysis summaries in two hours
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NextSeq 2000 Systems:
- High Output and Scalability: Offers an output range of 10 to 540 gigabases per run, supporting up to 1.8 billion single-end reads.
- Flexible Read Lengths: It supports read lengths from 1 × 50 base pairs to 2 × 300 base pairs, providing adaptability for different sequencing needs.
- Advanced XLEAP-SBS Chemistry: The system utilizes XLEAP-SBS chemistry, an enhanced version of Illumina’s sequencing-by-synthesis technology, offering faster, higher quality, and more robust sequencing.
- Integrated DRAGEN Bio-IT Platform: The NextSeq 2000 features onboard DRAGEN secondary analysis, providing rapid and accurate data processing directly on the instrument.
- Offers high accuracy for targeted and high-throughput sequencing, enabling wide-ranging genomic studies.
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SeqStudio Flex Series Genetic Analyzer:
The SeqStudio Flex Series Genetic Analyzer delivers cutting-edge solutions for DNA sequencing and fragment analysis. Designed for simplicity and reliability, it’s perfect for applications like:
- DNA Sequencing: High-accuracy results for mutation detection and genotyping.
- Fragment Analysis: Ideal for microsatellite studies, gene scanning, and HLA typing.
- Mutation Detection: Effortless SNP and indel identification.
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GridION ONT:
- Real-Time Analysis: Get immediate results as sequencing progresses.
- Scalability: Supports up to 5 MinION flow cells simultaneously for tailored output.
- Versatility: Perfect for whole-genome sequencing, transcriptomics, or targeted studies.
- Streamlined Workflow: Onboard compute for basecalling, analysis, and data management.