Kano Independent Research Centre Trust (KIRCT) – Genomics Centre

Genomics and Molecular Biology Lab:

This lab is equipped with state-of-the-art equipment for molecular biology techniques such as PCR, sequencing, and gene expression analysis.

Subunits:
  • Gene Expression Analysis
  •  Genotyping
  • Sequencing
Mission:

The Genomics Centre at Kano Independent Research Centre Trust (KIRCT) is committed to advancing the field of genomics in Nigeria, with a focus on improving public health and reducing the burden of diseases in the country. Our mission is to apply cutting-edge genomics technologies and expertise to address the pressing health challenges facing Nigeria, particularly in the areas of communicable and non-communicable diseases.

Objectives:

1. To establish a world-class genomics facility in Nigeria, equipped with state-of-the-art equipment and expertise.

2. To conduct research in genomics, focusing on preeclampsia, diabetes and hypertension prevalent in Nigeria.

3. To develop and implement genomic-based diagnostic tools and therapeutic strategies for the prevention and treatment of diseases.

4. To train and mentor local researchers, clinicians, and students in genomics research and its applications.

5. To collaborate with national and international organizations to advance genomics research and translation in Nigeria.

 

GENIE II Instrument

At the heart of our innovative genomic diagnostic lab, the GENIE II Instrument combines portability with precision, leveraging Loop-Mediated Isothermal Amplification (LAMP) technology to detect infectious diseases rapidly and reliably. Its user-friendly design and real-time data capabilities make it indispensable for field studies and laboratory diagnostics alike.

HPLC (High-Performance Liquid Chromatography)

The HPLC system is a benchmark for chemical and biological analysis precision. It is capable of isolating, identifying, and quantifying complex compounds. It serves as a critical tool for drug development, environmental analysis, and biomarker discovery.

LCMSD (Liquid Chromatography Mass Spectrometry Detector)

As a powerhouse for molecular profiling, the LCMSD system is used to explore intricate molecular interactions. It offers unparalleled accuracy and in-depth analysis, from drug metabolism studies to metabolomics.

NanoDrop Oneᶜ UV-Vis Spectrophotometer

The NanoDrop Oneᶜ is a microvolume marvel, quantifying nucleic acids and proteins with unparalleled precision using as little as 1-2 µL of sample. It maximizes data accuracy while minimizing sample waste.

Qubit™ Flex Fluorometer

The Qubit™ Flex Fluorometer offers highly accurate, sensitive quantification of nucleic acids, proteins, and other biomolecules. Complementing the nanodrop spectrophotometer. With the ability to process up to 8 samples simultaneously, it enhances high-throughput efficiency, making it ideal for genomic and proteomic research.

KingFisher Flex Automated Extraction System

High-throughput and highly efficient, the KingFisher Flex automates DNA and RNA purification processes for up to 96 samples per run. It ensures uniformity and reproducibility, saving precious time for researchers.

QuantStudio™ 7 Flex Real-Time PCR System

From gene expression to mutation analysis, the QuantStudio™ 7 Flex revolutionizes real-time PCR applications with precision and flexibility. Its multiplexing capabilities allow simultaneous analysis of multiple targets, supporting complex research endeavours.

Illumina MiSeq & NextSeq 2000 Sequencing Systems

The Illumina MiSeq is perfect for small-scale sequencing projects such as targeted gene sequencing, amplicon sequencing, and small RNA analysis, offering high accuracy and sensitivity for a variety of research needs. In contrast, the NextSeq 2000 provides a high-throughput solution, allowing for comprehensive transcriptomic and genomic sequencing. Whether we’re performing large-scale genome-wide association studies (GWAS) or in-depth transcriptome analyses, these systems support cutting-edge research in genomic medicine, population health, and precision diagnostics.

SeqStudio Flex Series Genetic Analyzer

Complementing our short-read sequencing capabilities, the SeqStudio Flex is a versatile platform for Sanger sequencing and fragment analysis. With exceptional throughput and accuracy, it is ideally suited for smaller-scale projects, genotyping, and sequence validation. This system is particularly effective for applications in clinical diagnostics, forensic science, and population genetics.

GridION ONT (Oxford Nanopore Technologies)

For long-read sequencing, the GridION ONT offers a revolutionary solution for high-quality, real-time DNA sequencing. Its ability to read long stretches of DNA without the need for amplification enables structural variant detection, epigenetic analysis, and the sequencing of entire genomes with unprecedented resolution. This long-read capability complements our short-read systems and expands the scope of our research to include complex genomic regions that are typically difficult to sequence with traditional methods.                                                                                                              

A Unified, Powerful Sequencing Ecosystem

By seamlessly integrating these solar-powered systems, KIRCT is equipped to perform a wide variety of genomic analyses, from small-scale targeted studies to large, complex genomic investigations. Our lab is uniquely positioned to offer high-throughput sequencing with the flexibility to conduct both short-read and long-read analyses. This versatility enables us to tackle a broad spectrum of research challenges, including disease genomics, precision medicine, pathogen surveillance, and population genomics.